CARDIOPROTECTIVE POTENTIAL OF CONVOLVULUS ARVENSIS AGAINST ISOPROTERENOL-INDUCED CARDIOTOXICITY
DOI:
https://doi.org/10.69723/njms.04.03.0613Keywords:
Cardiotoxicity, Cardioprotection, Convolvulus arvensis, Isoproterenol, Myocardial infarction, Oxidative stressAbstract
BACKGROUND: Myocardial infarction is largely driven by oxidative stress-mediated cellular and mitochondrial dysfunction. Isoproterenol-based experimental models closely mimic these pathological mechanisms, while plant-derived antioxidants have gained recognition as potential cardioprotective agents against oxidative injury.
OBJECTIVE: This study aimed to investigate the cardioprotective effects of methanolic extract from Convolvulus arvensis against Isoproterenol-induced cardiotoxicity in rats, focusing on myocyte injury markers and histopathological changes.
METHODOLOGY: The experimental study was carried out in the Department of Pharmacology at Khyber Medical College and Khyber Medical University, Peshawar, Pakistan, between August 2022 and April 2023. Female Sprague Dawley rats were randomly allocated into three main groups: a positive control, a negative control, and treatment groups receiving Convolvulus arvensis extract at doses of 50 mg/kg, 150 mg/kg, and 300 mg/kg orally for 14 days. Myocardial infarction was induced in rats by administering Isoproterenol (100mg/kg SC) on the 13th and 14th days. Biochemical and histopathological assessments were conducted on the 15th day.
RESULTS: Administration of Isoproterenol led to changes in serum levels of cardiac injury markers (CK-MB and LDH) and histopathological alterations. Pre-treatment with Convolvulus arvensis extract prevented all parameters of Isoproterenol-induced myocardial infarction in rats, as confirmed by histopathological examination. The study findings suggest that Convolvulus arvensis exerts significant protective effects on the heart against Isoproterenol-induced myocardial infarction by maintaining endogenous antioxidant enzyme activities.
CONCLUSION: Convolvulus arvensis extract significantly lowered serum CK-MB levels and mitigated histopathological damage in isoproterenol-treated rats, refelecting its potential as a cardioprotective agent.
KEYWORDS: Cardiotoxicity, Cardioprotection, Convolvulus arvensis, Isoproterenol, Myocardial infarction, Oxidative stress
References
Singh AK, Jat RK. Myocardial infarction. Himal J Health Sci. 2021;6:16-32. https://doi.org/10.22270/hjhs.v6i4.116.
Jomova K, Raptova R, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, et al. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Arch. Toxicol. 2023;97(10):2499-574. https://doi.org/10.1007/s00204-023-03562-9.
Juan CA, Pérez de la Lastra JM, Plou FJ, Pérez-Lebeña E. The chemistry of reactive oxygen species (ROS) revisited: outlining their role in biological macromolecules (DNA, lipids and proteins) and induced pathologies. Int J Mol Sci. 2021;22(9):4642. https://doi.org/10.3390/ijms22094642.
Anik MI, Mahmud N, Masud AA, Khan MI, Islam MN, Uddin S, et al. Role of reactive oxygen species in aging and age-related diseases: a review. ACS Appl. Bio Mater. 2022;5(9):4028-54. https://doi.org/10.1021/acsabm.2c00411.
Liu M, Liu P, Zheng B, Liu Y, Li L, Han X, et al. Cardioprotective effects of alantolactone on isoproterenol-induced cardiac injury and cobalt chloride-induced cardiomyocyte injury. Int J Immunopathol Pharmacol. 2022;36. https://doi.org/10.1177/20587384211051993.
Anamalley R, Rajassageran L, Apparoo Y, Jauri MH, Kamisah Y, Yunos NM, et al. Repeated administration of low dose isoprenaline on the rat’s cardiovascular system. Sains Malays. 2022;51(7):2147-57. http://doi.org/10.17576/jsm-2022-5107-17.
Chainy GB, Sahoo DK. Hormones and oxidative stress: an overview. Free Radic Res. 2020;54(1):1-26. http://doi.org/10.1080/10715762.2019.1702656.
Bajaj S, Singh S, Sharma P. Role of antioxidants in neutralizing oxidative stress. Nutraceutical fruits and foods for neurodegenerative disorders: Elsevier; 2024. p. 353-78. https://doi.org/10.1016/B978-0-443-18951-7.00020-7.
Pandi A, Raghu MH, Chandrashekar N, Kalappan VM. Cardioprotective effects of Ferulic acid against various drugs and toxic agents. Beni-Suef univ j basic appl sci. 2022;11(1):92. https://doi.org/10.1186/s43088-022-00273-5.
Sajid A, Ahmad T, Ikram M, Khan T, Shah AJ, Mahnashi MH, et al. Cardioprotective Potential of Aqueous Extract of Fumaria indica on Isoproterenol-Induced Myocardial Infarction in SD Rats. Oxid Med Cell Longev. 2022(1):2112956. https://doi.org/10.1155/2022/2112956.
Ojha S, Golechha M, Kumari S, Arya DS. Protective effect of Emblica officinalis (amla) on isoproterenol-induced cardiotoxicity in rats. Toxicol Ind Health. 2012;28(5):399-411. https://doi.org/10.1177/0748233711413798.
Narayanan G, Prabhu K, Chaudhury AB, Rao MRK, Selvi VSK, Muthiah NS, et al. Cardioprotective role of Partharishtam on isopreterenol-induced myocardial infarction in animal model. Pharmacogn J. 2021;13(2):591–5. doi:10.5530/pj.2021.13.74
Salamatullah AM. Convolvulus arvensis: antioxidant, antibacterial, and antifungal properties of chemically profiled essential oils: an approach against nosocomial infections. Life. 2022;12(12):2138. https://doi.org/10.3390/life12122138.
Mosallaie F, Pirnia M, Dehghan Z, Falah F, Sabbaghzadeh R, Behbahani BA, et al. Unveiling the chemical composition, antioxidant and antibacterial properties, and mechanistic insights of Convolvulus arvensis extract through molecular docking simulations. Appl Food Res. 2024;4(2):100580. https://doi.org/10.1016/j.afres.2024.100580.
Flori L, Lazzarini G, Spezzini J, Pirone A, Calderone V, Testai L, et al. The isoproterenol-induced myocardial fibrosis: A biochemical and histological investigation. Biomed Pharmacother. 2024;174:116534. https://doi.org/10.1016/j.biopha.2024.116534.
Thakral J, Borar S, Kalia A. Antioxidant potential fractionation from methanol extract of aerial parts of Convolvulus arvensis Linn.(Convolvulaceae). Int J Pharm Sci Drug Res. 2010;2(3):219-23.
Ali M, Qadir MI, Saleem M, Janbaz KH, Gul H, Hussain L, et al. Hepatoprotective potential of Convolvulus arvensis against paracetamol-induced hepatotoxicity. Bangladesh J Pharmacol. 2013;8(3):300-4. https://doi.org/10.3329/bjp.v8i3.15165.
Panda S, Kar A, Biswas S. Preventive effect of Agnucastoside C against Isoproterenol-induced myocardial injury. Sci Rep. 2017;7(1):16146. https://doi.org/10.1038/s41598-017-16075-0.
Shukla SK, Sharma SB, Singh UR. β-Adrenoreceptor agonist isoproterenol alters oxidative status, inflammatory signaling, injury markers and apoptotic cell death in myocardium of rats. Indian J Clin Biochem. 2015;30(1):27-34. https://doi.org/10.1007/s12291-013-0401-5.
Farag MM, Khalifa AA, Elhadidy WF, Rashad RM. Thymoquinone dose-dependently attenuates myocardial injury induced by isoproterenol in rats via integrated modulations of oxidative stress, inflammation, apoptosis, autophagy, and fibrosis. Naunyn-Schmiedeberg’s Archives of Pharmacology. 2021 Aug;394(8):1787–1801. doi:10.1007/s00210-021-02087-1.
Lobo Filho HG, Ferreira NL, Sousa RBd, Carvalho ERd, Lobo PLD, Lobo Filho JG. Experimental model of myocardial infarction induced by isoproterenol in rats. Braz J Cardiovasc Surg. 2011;26:469-76. https://doi.org/10.5935/1678-9741.20110024.
Garg M, Khanna D. Exploration of pharmacological interventions to prevent isoproterenol-induced myocardial infarction in experimental models. Ther Adv Cardiovasc Dis. 2014;8(4):155-69. https://doi.org/10.1177/1753944714531638.
Xiang M, Lu Y, Xin L, Gao J, Shang C, Jiang Z, et al. Role of oxidative stress in reperfusion following myocardial ischemia and its treatments. Oxid Med Cell Longev. 2021;2021(1):6614009. https://doi.org/10.1155/2021/6614009.
Enayati A, Hatemi BMJ, Pullaiah T. Functional Plants/Nutraceuticals as Myocardial Ischemia/Reperfusion Protective Agents. Cardioprotective Plants: Springer; 2024. p. 233-61. https://doi.org/10.1007/978-981-97-4627-9_8.
Attia AA, Sorour JM, Mohamed NA, Mansour TT, Al-Eisa RA, El-Shenawy NS. Biochemical, histological, and ultrastructural studies of the protective role of vitamin E on cyclophosphamide-induced cardiotoxicity in male rats. Biomedicines. 2023;11(2):390. https://doi.org/10.3390/biomedicines11020390.
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